{"id":90,"date":"2023-07-26T11:16:16","date_gmt":"2023-07-26T11:16:16","guid":{"rendered":"https:\/\/helixlab.com.tr\/?page_id=90"},"modified":"2025-08-29T07:11:32","modified_gmt":"2025-08-29T07:11:32","slug":"hucre-kulturu","status":"publish","type":"page","link":"https:\/\/helixlab.com.tr\/?page_id=90","title":{"rendered":"H\u00fccre K\u00fclt\u00fcr\u00fc"},"content":{"rendered":"\t\t
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H\u00dcCRE K\u00dcLT\u00dcR\u00dc<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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H\u00dcCRE K\u00dcLT\u00dcR\u00dc<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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HelixLab H\u00fccre K\u00fclt\u00fcr\u00fc Laboratuvar\u0131, ara\u015ft\u0131rma projelerine destek sa\u011flaman\u0131n yan\u0131 s\u0131ra<\/strong>, tez d\u00f6nemi \u00f6\u011frencilerine ve stajyerlerimize uygulamal\u0131 e\u011fitimler<\/strong> sunarak \u201cbilim i\u00e7in bilim\u201d anlay\u0131\u015f\u0131n\u0131 desteklemektedir.<\/p>

Laboratuvar\u0131m\u0131zda aktif olarak kullan\u0131lan ba\u015fl\u0131ca cihazlar:<\/p>

  • Biorad TC20-Cell Counter<\/strong> \u2013 H\u00fccre say\u0131m\u0131 ve analizleri i\u00e7in<\/p><\/li>

  • ThermoFisher Evos XL Core Mikroskop<\/strong> \u2013 H\u00fccre g\u00f6zlemleri ve g\u00f6r\u00fcnt\u00fcleme \u00e7al\u0131\u015fmalar\u0131 i\u00e7in<\/p><\/li><\/ul>

    Bu altyap\u0131 sayesinde laboratuvar\u0131m\u0131z, \u00e7e\u015fitli h\u00fccre tiplerinin in vitro k\u00fclt\u00fcrlerini ger\u00e7ekle\u015ftirerek<\/strong>, ara\u015ft\u0131rmac\u0131lar\u0131n kanser, k\u00f6k h\u00fccre ve h\u00fccre mekani\u011fi gibi alanlarda h\u00fccre i\u00e7i ve h\u00fccreler aras\u0131 biyokimyasal s\u00fcre\u00e7leri molek\u00fcler d\u00fczeyde incelemelerine<\/strong> olanak sa\u011flamaktad\u0131r.<\/p>

    T\u00fcm \u00e7al\u0131\u015fmalar\u0131m\u0131z ara\u015ft\u0131rma ve Ar-Ge projeleri odakl\u0131d\u0131r<\/strong>; tan\u0131 veya klinik tedavi ama\u00e7l\u0131 de\u011fildir.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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    Memeli H\u00fccre K\u00fclt\u00fcr\u00fc<\/strong><\/h4>

    H\u00fccre k\u00fclt\u00fcr\u00fc, h\u00fccrelerin kontroll\u00fc laboratuvar ko\u015fullar\u0131nda yeti\u015ftirilmesi ve \u00e7o\u011falt\u0131lmas\u0131<\/strong> s\u00fcrecidir. Bu y\u00f6ntem sayesinde, canl\u0131 organizmalarda (in vivo) yap\u0131lamayan deneyler in vitro ortamda<\/strong> ger\u00e7ekle\u015ftirilebilir ve bilimsel sonu\u00e7lara ula\u015f\u0131labilir.<\/p>

    HelixLab H\u00fccre K\u00fclt\u00fcr\u00fc Laboratuvar\u0131\u2019nda y\u00fcr\u00fct\u00fclen \u00e7al\u0131\u015fmalar aras\u0131nda \u015funlar yer almaktad\u0131r:<\/p>

    • Memeli h\u00fccrelerinin in vitro \u00fcretimi<\/p><\/li>

    • Sitotoksik ve apoptotik etkilerin ara\u015ft\u0131r\u0131lmas\u0131<\/p><\/li>

    • H\u00fccre manip\u00fclasyonu ve protein analizleri<\/p><\/li>

    • K\u00f6k h\u00fccrelerin in vitro deneyleri ve farkl\u0131la\u015ft\u0131rmalar\u0131<\/p><\/li><\/ul>

      T\u00fcm bu \u00e7al\u0131\u015fmalar ara\u015ft\u0131rma, geli\u015ftirme ve Ar-Ge projelerine destek amac\u0131yla<\/strong> yap\u0131lmakta olup, tan\u0131 veya klinik tedavi ama\u00e7l\u0131 de\u011fildir.<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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      Doku K\u00fclt\u00fcr\u00fc<\/strong><\/h4>

      Doku k\u00fclt\u00fcr\u00fc, dokular\u0131n ve h\u00fccrelerin canl\u0131 organizman\u0131n d\u0131\u015f\u0131nda (ex vivo)<\/strong>, s\u0131v\u0131, yar\u0131-kat\u0131 veya kat\u0131 besiyerlerinde yeti\u015ftirilmesi s\u00fcrecidir.<\/p>

      Bu y\u00f6ntem, h\u00fccre k\u00fclt\u00fcr\u00fc ile ayn\u0131 anlamda kullan\u0131labilece\u011fi gibi, canl\u0131dan al\u0131nan doku par\u00e7alar\u0131n\u0131n in vitro olarak yeti\u015ftirilmesi<\/strong> anlam\u0131na da gelmektedir.<\/p>

      HelixLab\u2019da y\u00fcr\u00fct\u00fclen doku k\u00fclt\u00fcr\u00fc \u00e7al\u0131\u015fmalar\u0131 ile:<\/p>

      • H\u00fccreler ve dokular belirlenen kontroll\u00fc ko\u015fullar alt\u0131nda<\/strong> tutulmakta,<\/p><\/li>

      • \u0130stenilen deneysel uygulamalar yap\u0131lmakta,<\/p><\/li>

      • Sonu\u00e7lar daha objektif ve tekrarlanabilir<\/strong> \u015fekilde izlenebilmektedir.<\/p><\/li><\/ul>

        Bu nedenle doku k\u00fclt\u00fcr\u00fc, h\u00fccre biyolojisi ve molek\u00fcler ara\u015ft\u0131rmalarda<\/strong> g\u00fcn\u00fcm\u00fczde kritik bir y\u00f6ntem olarak kullan\u0131lmaktad\u0131r.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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        H\u00fccre \u00c7o\u011faltma, Dondurma, Pasajlama<\/strong><\/h4>

        HelixLab\u2019da y\u00fcr\u00fct\u00fclen h\u00fccre k\u00fclt\u00fcr\u00fc \u00e7al\u0131\u015fmalar\u0131 kapsam\u0131nda:<\/p>

        • Pasajlama:<\/strong> Primer k\u00fclt\u00fcr kab\u0131ndaki h\u00fccreler b\u00fcy\u00fcy\u00fcp mevcut kab\u0131 doldurdu\u011funda, h\u00fccrelerin s\u00fcrekli b\u00fcy\u00fcmesini sa\u011flamak ve deneylerin s\u00fcreklili\u011fini korumak amac\u0131yla pasaj i\u015flemi uygulan\u0131r.<\/p><\/li>

        • Donmu\u015f H\u00fccrelerin Kullan\u0131m\u0131:<\/strong> Daha \u00f6nce dondurulmu\u015f h\u00fccreler, ara\u015ft\u0131rma veya \u00fcretim projelerinde yeni k\u00fclt\u00fcrler olu\u015fturmak i\u00e7in \u00e7\u00f6zd\u00fcr\u00fcl\u00fcr<\/strong>.<\/p><\/li>

        • \u00c7\u00f6zd\u00fcrme \u0130\u015flemi:<\/strong> Donmu\u015f h\u00fccrelerin \u00e7\u00f6zd\u00fcr\u00fclmesi, dondurma kadar \u00f6zen gerektiren kritik bir ad\u0131md\u0131r; bu s\u00fcre\u00e7, h\u00fccre sa\u011fl\u0131\u011f\u0131n\u0131n ve deneylerin g\u00fcvenilirli\u011finin korunmas\u0131 a\u00e7\u0131s\u0131ndan \u00f6nemlidir.<\/p><\/li><\/ul>

          T\u00fcm bu uygulamalar, ara\u015ft\u0131rma ve Ar-Ge projeleri kapsam\u0131nda<\/strong> ger\u00e7ekle\u015ftirilmektedir; tan\u0131 veya klinik tedavi ama\u00e7l\u0131 de\u011fildir.<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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          H\u00fccre Canl\u0131l\u0131k Analizleri<\/strong><\/h4>

          HelixLab\u2019da h\u00fccre morfolojisi ve canl\u0131l\u0131k analizleri i\u00e7in boyama teknikleri<\/strong> uygulanmaktad\u0131r. Bu y\u00f6ntemler, h\u00fccrelerin mikroskop alt\u0131nda g\u00f6zlemlenmesi ve karakterize edilmesi<\/strong> amac\u0131yla kullan\u0131l\u0131r.<\/p>

          • Canl\u0131\/\u00d6l\u00fc H\u00fccre Ayr\u0131m\u0131:<\/strong> H\u00fccre membran b\u00fct\u00fcnl\u00fc\u011f\u00fc korunmu\u015f h\u00fccreler boyay\u0131 almazken, membran\u0131 hasar g\u00f6rm\u00fc\u015f \u00f6l\u00fc h\u00fccreler boya ile boyan\u0131r.<\/p><\/li>

          • Kullan\u0131lan Boyalar:<\/strong> Eritrosin-B, trypan mavisi gibi boyalar kullan\u0131larak \u00f6l\u00fc h\u00fccreler sitoplazmalar\u0131n\u0131n boyanmas\u0131 ile ay\u0131rt edilir, canl\u0131 h\u00fccreler ise boyay\u0131 almad\u0131\u011f\u0131 i\u00e7in parlak renkte g\u00f6zlemlenir.<\/p><\/li><\/ul>

            Bu teknikler, ara\u015ft\u0131rma ve Ar-Ge projelerinde h\u00fccre sa\u011fl\u0131\u011f\u0131n\u0131n izlenmesi, deneylerin g\u00fcvenilirli\u011finin art\u0131r\u0131lmas\u0131 ve yeni y\u00f6ntemlerin geli\u015ftirilmesi<\/strong> amac\u0131yla y\u00fcr\u00fct\u00fclmektedir.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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            Sitotoksisite Analizleri<\/strong><\/h4>

            HelixLab\u2019da y\u00fcr\u00fct\u00fclen h\u00fccre bazl\u0131 \u00e7al\u0131\u015fmalar kapsam\u0131nda, biyouyumluluk, implantlar, kozmetik \u00fcr\u00fcnler, farmakoloji ve ila\u00e7 geli\u015ftirme projeleri<\/strong>, toksik ajanlar\u0131n etkilerinin ara\u015ft\u0131r\u0131lmas\u0131 ve antikanser ila\u00e7lar\u0131n\u0131n h\u00fccre d\u00fczeyindeki etkilerinin incelenmesi gibi ama\u00e7larla sitotoksisite testleri<\/strong> uygulanmaktad\u0131r.<\/p>

            H\u00fccre canl\u0131l\u0131\u011f\u0131n\u0131n veya sitotoksisitenin belirlenmesi i\u00e7in kullan\u0131lan ba\u015fl\u0131ca y\u00f6ntemler:<\/p>

            • Boyama Y\u00f6ntemleri<\/strong> \u2013 H\u00fccre canl\u0131l\u0131\u011f\u0131 ve morfolojisi \u00fczerine analizler<\/p><\/li>

            • Kolorimetrik Y\u00f6ntemler<\/strong> \u2013 H\u00fccre metabolik aktivitesinin \u00f6l\u00e7\u00fclmesi<\/p><\/li>

            • Florometrik Y\u00f6ntemler<\/strong> \u2013 Floresan temelli canl\u0131l\u0131k ve stres \u00f6l\u00e7\u00fcmleri<\/p><\/li>

            • Luminometrik Y\u00f6ntemler<\/strong> \u2013 I\u015f\u0131k temelli metabolik ve sitotoksisite \u00f6l\u00e7\u00fcmleri<\/p><\/li>

            • Apoptoz Analizleri<\/strong> \u2013 Programl\u0131 h\u00fccre \u00f6l\u00fcm\u00fcn\u00fcn belirlenmesi<\/p><\/li>

            • Otofaji Analizleri<\/strong> \u2013 H\u00fccre i\u00e7i geri d\u00f6n\u00fc\u015f\u00fcm ve metabolik s\u00fcre\u00e7lerin incelenmesi<\/p><\/li><\/ul>

              T\u00fcm bu testler, ara\u015ft\u0131rma ve Ar-Ge projelerine destek amac\u0131yla y\u00fcr\u00fct\u00fclmekte olup<\/strong>, tan\u0131 veya klinik tedavi amac\u0131 ta\u015f\u0131mamaktad\u0131r.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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              H\u00fccresel D\u00fczeyde Antikanser Aktivitenin Belirlenmesi<\/strong><\/h4>

              HelixLab\u2019da, anti-kanser etkili yeni molek\u00fcllerin sentezlenmesi ve ara\u015ft\u0131r\u0131lmas\u0131<\/strong>, modern biyoteknoloji ve ila\u00e7 geli\u015ftirme projeleri kapsam\u0131nda y\u00fcr\u00fct\u00fclmektedir.<\/p>

              Bu \u00e7al\u0131\u015fmalar, kanser h\u00fccrelerinin biyolojik mekanizmalar\u0131n\u0131 anlamak, potansiyel etken maddeleri belirlemek ve ara\u015ft\u0131rma protokollerini geli\u015ftirmek<\/strong> amac\u0131yla ger\u00e7ekle\u015ftirilir. Laboratuvar\u0131m\u0131z, di\u011fer ara\u015ft\u0131rma gruplar\u0131yla benzer \u015fekilde, ara\u015ft\u0131rma ve Ar-Ge projelerinde anti-kanser potansiyeli olan yeni bile\u015fenlerin ke\u015ffine odaklanmaktad\u0131r.<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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              Wound Healing Testi<\/strong><\/h4>

              Yara, dokular\u0131n b\u00fct\u00fcnl\u00fc\u011f\u00fcn\u00fcn ve i\u015flevinin bozulmas\u0131 olarak tan\u0131mlan\u0131r. Dokular\u0131n eski i\u015fleyi\u015fine ve b\u00fct\u00fcnl\u00fc\u011f\u00fcne kavu\u015fma s\u00fcreci ise yara iyile\u015fmesi<\/strong> olarak adland\u0131r\u0131l\u0131r.<\/p>

              • Akut Yaralar:<\/strong> Travmatik veya cerrahi nedenlerle olu\u015fan, genellikle iyile\u015fmeye iyi yan\u0131t veren ve temiz yaralard\u0131r.<\/p><\/li>

              • Kronik Yaralar:<\/strong> Ge\u00e7 iyile\u015fen veya iyile\u015fmeyen yaralard\u0131r. Diyabetik ayak, ven\u00f6z \u00fclser, bas\u0131n\u00e7 yaras\u0131, arteriyel yetmezlik yaras\u0131 ve radyasyon hasar\u0131 kronik yaralar\u0131n s\u0131k g\u00f6r\u00fclen nedenlerindendir.<\/p><\/li><\/ul>

                HelixLab H\u00fccre ve Doku Laboratuvar\u0131\u2019nda, h\u00fccre g\u00f6\u00e7\u00fc ve h\u00fccre-h\u00fccre etkile\u015fimlerini inceleyen teknikler<\/strong> kullan\u0131larak yara iyile\u015fme s\u00fcre\u00e7leri ara\u015ft\u0131r\u0131lmakta ve yara iyile\u015fmesini h\u0131zland\u0131rmak amac\u0131yla geli\u015ftirilen \u00fcr\u00fcnlerin Ar-Ge \u00e7al\u0131\u015fmalar\u0131<\/strong> y\u00fcr\u00fct\u00fclmektedir.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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                Transfeksiyon<\/strong><\/h4>

                Transfeksiyon, yabanc\u0131 n\u00fckleik asitlerin \u00f6karyotik h\u00fccrelere aktar\u0131lmas\u0131<\/strong> ve bu sayede genetik olarak modifiye edilmi\u015f h\u00fccrelerin olu\u015fturulmas\u0131<\/strong> s\u00fcrecidir.<\/p>

                HelixLab\u2019da transfeksiyon teknikleri, gen fonksiyonu, reg\u00fclasyonu ve protein fonksiyonlar\u0131n\u0131n ara\u015ft\u0131r\u0131lmas\u0131<\/strong> gibi \u00e7al\u0131\u015fmalarda g\u00fc\u00e7l\u00fc bir analitik ara\u00e7 olarak kullan\u0131lmaktad\u0131r.<\/p>

                T\u00fcm uygulamalar, ara\u015ft\u0131rma ve geli\u015ftirme projelerine destek<\/strong> amac\u0131yla y\u00fcr\u00fct\u00fclmekte olup, tan\u0131 veya klinik tedavi amac\u0131 ta\u015f\u0131mamaktad\u0131r.<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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                Genom Toksisite, Metilasyon \u00c7al\u0131\u015fmalar\u0131<\/strong><\/h4>

                Genetik toksisite, genotoksinlerin DNA ve kromozom yap\u0131s\u0131nda meydana getirdi\u011fi hasarlar\u0131<\/strong> tan\u0131mlayan bir terimdir. Bu hasarlar; gen mutasyonlar\u0131, kromozomal anormallikler, DNA zincir k\u0131r\u0131klar\u0131 ve DNA eklentileri gibi yap\u0131sal de\u011fi\u015fiklikleri kapsar.<\/p>

                HelixLab\u2019da uygulanan genotoksisite testleri<\/strong>, kimyasal ve fiziksel ajanlar\u0131n genotoksik potansiyelini ara\u015ft\u0131rmak amac\u0131yla kullan\u0131lmaktad\u0131r. Bu testler sayesinde:<\/p>

                • Fiziksel etkenler, ila\u00e7lar, \u00e7evresel kirleticiler ve g\u0131da katk\u0131 maddeleri gibi g\u00fcnl\u00fck ya\u015famda maruz kal\u0131nan maddelerin genotoksik ve potansiyel kanserojen etkileri<\/strong>,<\/p><\/li>

                • Bu maddelerin biyolojik g\u00fcvenilirli\u011fi ve toksisite profilleri<\/strong>,<\/p><\/li>

                • Kanser riskini \u00f6ng\u00f6rmeye y\u00f6nelik biyolojik izlem \u00e7al\u0131\u015fmalar\u0131<\/strong><\/p><\/li><\/ul>

                  ara\u015ft\u0131rma ve Ar-Ge projeleri kapsam\u0131nda y\u00fcr\u00fct\u00fclmektedir.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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                  \n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\"\"\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

                  H\u00dcCRE K\u00dcLT\u00dcR\u00dc H\u00dcCRE K\u00dcLT\u00dcR\u00dc HelixLab H\u00fccre K\u00fclt\u00fcr\u00fc Laboratuvar\u0131, ara\u015ft\u0131rma projelerine destek sa\u011flaman\u0131n yan\u0131 s\u0131ra, tez d\u00f6nemi \u00f6\u011frencilerine ve stajyerlerimize uygulamal\u0131 e\u011fitimler sunarak \u201cbilim i\u00e7in bilim\u201d…<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-90","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/helixlab.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/90","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/helixlab.com.tr\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/helixlab.com.tr\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/helixlab.com.tr\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/helixlab.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=90"}],"version-history":[{"count":5,"href":"https:\/\/helixlab.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/90\/revisions"}],"predecessor-version":[{"id":765,"href":"https:\/\/helixlab.com.tr\/index.php?rest_route=\/wp\/v2\/pages\/90\/revisions\/765"}],"wp:attachment":[{"href":"https:\/\/helixlab.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=90"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}